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mir eclip sequencing  (Eclipse Bioinnovations)
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Eclipse Bioinnovations mir eclip sequencing
( A ). Plot of genes with mRNA transcripts targeted by <t>both</t> <t>hsa-miR-28-5p</t> and hsa-miR-708-5p. The <t>miR-eCLIP</t> data were filtered for genes expressed in MCF10A cells, and RNAseq differential expression data following MIR-28 treatment is graphed for each gene on the x- and y-axes. Bolded points represent genes downregulated by both MIR-28 mimics by at least -0.5 log2 units, with a differential expression false discovery rate < 0.05 for both treatments. All genes are labeled with their HGNC symbol. ( B ). Plot of genes with mRNA transcripts targeted exclusively (XOR) by either hsa-miR-28-5p or hsa- miR-708-5p. The miR-eCLIP data were filtered and graphed as above. The genes encoding mRNA transcripts are colored blue if bound by hsa-miR-28-5p or red if bound by hsa-miR-708-5p, according to miR-eCLIP data. Select genes are labeled with HGNC symbol.
Mir Eclip Sequencing, supplied by Eclipse Bioinnovations, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mir+eclip+sequencing/miR-eCLIP/bio_rxiv__2023__02__17__526327-141-15-20
Average 94 stars, based on 1 article reviews
mir eclip sequencing - by Bioz Stars, 2026-11
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( A ). Plot of genes with mRNA transcripts targeted by both hsa-miR-28-5p and hsa-miR-708-5p. The miR-eCLIP data were filtered for genes expressed in MCF10A cells, and RNAseq differential expression data following MIR-28 treatment is graphed for each gene on the x- and y-axes. Bolded points represent genes downregulated by both MIR-28 mimics by at least -0.5 log2 units, with a differential expression false discovery rate < 0.05 for both treatments. All genes are labeled with their HGNC symbol. ( B ). Plot of genes with mRNA transcripts targeted exclusively (XOR) by either hsa-miR-28-5p or hsa- miR-708-5p. The miR-eCLIP data were filtered and graphed as above. The genes encoding mRNA transcripts are colored blue if bound by hsa-miR-28-5p or red if bound by hsa-miR-708-5p, according to miR-eCLIP data. Select genes are labeled with HGNC symbol.

Journal: bioRxiv

Article Title: Discovery of novel microRNA regulators of ribosome biogenesis

doi: 10.1101/2023.02.17.526327

Figure Lengend Snippet: ( A ). Plot of genes with mRNA transcripts targeted by both hsa-miR-28-5p and hsa-miR-708-5p. The miR-eCLIP data were filtered for genes expressed in MCF10A cells, and RNAseq differential expression data following MIR-28 treatment is graphed for each gene on the x- and y-axes. Bolded points represent genes downregulated by both MIR-28 mimics by at least -0.5 log2 units, with a differential expression false discovery rate < 0.05 for both treatments. All genes are labeled with their HGNC symbol. ( B ). Plot of genes with mRNA transcripts targeted exclusively (XOR) by either hsa-miR-28-5p or hsa- miR-708-5p. The miR-eCLIP data were filtered and graphed as above. The genes encoding mRNA transcripts are colored blue if bound by hsa-miR-28-5p or red if bound by hsa-miR-708-5p, according to miR-eCLIP data. Select genes are labeled with HGNC symbol.

Article Snippet: Approximately 10 million crosslinked cells from each microRNA mimic treatment were pooled and processed for miR-eCLIP sequencing and data analysis (Eclipse Bioinnovations, San Diego, CA).

Techniques: Expressing, Labeling